5/6/2023 0 Comments Cl molar massPre- and post-eruptive enamel on murine incisors only differed significantly in At%Ca ( p = 0.041) and At%P ( p = 0.026) with both At% higher in the pre-eruptive enamel. WDX revealed iron in the outer 10 µm of pre- and post-eruptive enamel of murine incisors. Enamel from murine molars had a higher Ca/P-Ratio than murine incisors and the highest Ca/P-Ratio in dentin was observed for human teeth and murine molars (1.49). The Ca/P-Ratio was the highest in human and bovine enamel, which did not differ significantly. Significant differences between human and bovine teeth were found for nitrogen in dentin, with a median of 4.5 (3.3–5) At%N for human dentin and 2.7 (2.3–3.2) At%N for bovine dentin. In dentin, murine teeth revealed a higher At%Ca compared to human and bovine teeth. Bovine and human enamel and dentin revealed close similarities regarding elemental composition. The presence of iron in murine incisor enamel was investigated using additional wavelength dispersive X-ray spectroscopy measurements (WDX) near the enamel surface. Furthermore, Ca/P- and Ca/N-Ratios were calculated. EDX-field scans and line scans were analyzed for elements Ca, P, O, C, N, F, Na, Mg, Fe, Cl, and S. Six human molars, bovine incisors, murine incisors, and murine molars were analyzed. In vitro, energy dispersive X-ray spectroscopy (EDX), improved by calibration standards and Si 3N 4-window material, enables determining local elemental compositions of inorganic and organic compounds without sample destruction. Teeth with different chemical compositions can show vastly different physical properties, so knowledge of elemental composition is required to use animal teeth as substitutes for human teeth in research.
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